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. 2009 Apr 29;4(4):e5368. doi: 10.1371/journal.pone.0005368

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) The promoter was amplified from BM and BM-IVGT, but not from BMΔvirB, showing that the promoter was deleted in BMΔvirB and complemented in BM-IVGT. B) RT-PCR amplification of virB1 and virB8 showed that the two genes could be amplified from BM and BM-IVGT, but not BMΔvirB, indicating that transcription of virB genes was inactivated in BMΔvirB and restored in BM-IVGT.