Figure 2. Determination of in vitro induction conditions of virB.
A: Transcription of virB under different in vitro condition. BM was firstly cultured in TSB to logarithmic phase and then subjected to different stresses. RNA was isolated and transcription of virB was quantified by qRT-PCR. virB was greatly activated under GEM4.0. B: Transcription of virB at different incubation time in GEM4.0. BM was subjected to different incubation time in GEM4.0 and then transcription of virB was quantified. The virB was greatly activated at 3 h.
