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. 2009 Apr 29;4(4):e5368. doi: 10.1371/journal.pone.0005368

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Spot distribution of the selected virulence related protein on gel of BM. Several protein spots with different MW and pI of omp25 were identified. B: Relative transcription of virulence related genes in BM and BMΔvirB. BM and BMΔvirB were firstly cultured in TSB to logarithmic phase and then transferred into GEM4.0 for 3 h. RNA was isolated and relative transcription of virulence related genes was quantified by normalization with 16S rRNA. These genes were transcribed at a lower level in BMΔvirB than in BM.