Figure 5. Transcriptional profile of virB during host cell infection.

Macrophage like cell J774A.1 were infected with BM, BMΔvirB and BM-IVGT. At different time (0, 12, 24 and 48 h) post the infection, RNA was isolated from the infection mixtures and reverse transcribed into cDNA. Transcription of virB genes were then quantified by qRT-PCR. A: Relative transcription of virB1 and virB8. The virB1 and virB8 was identically transcribed. B: Relative transcription of virB8 in BM, BMΔvirB and BM-IVGT. Transcription of virB8 peaked at 12 h and then decreased in BM and BM-IVGT. No transcription of virB8 was detected in BMΔvirB and uninfected macrophage cells.