Figure 8. Receptor expression, and effect of IFNγ on gene expression in CF-TG cells.

A, wt TG and CF-TG cells were incubated in the absence or presence of P. aeruginosa-secreted products for 24 h. The membrane expression of IFNγR2 and TLR2 was determined by flow cytometry. Histograms are representative of three different experiments. Hatched line, control IgG; Full line, unstimulated cells; Grey color, cells stimulated with P. aeruginosa-secreted products. B, 1000 UI of IFNγ were added to wt TG cells (1) and CF-TG cells (2) for 3 h, and the expression of genes encoding IFNγR2, Il-24 and CXCL10 was studied by qRT-PCR. Results, which represent means±SEM of three experiments, are expressed as fold change. (1) IFNγ-treated vs. untreated wt TG cells; (2) IFNγ-treated vs. untreated CF-TG cells.