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. 2009 Apr 29;4(4):e5391. doi: 10.1371/journal.pone.0005391

Figure 5. FX06 prevents thrombin-induced RhoA activation.

Figure 5

Endothelial cells were exposed to thrombin (1 U/ml), FX06 (50 µg/ml) or thrombin plus FX06 for indicated times. Pull down experiments were performed with GST-coupled Rhotekin (for activated RhoA) or with GST-coupled Pak-1 (for activated Rac1). Graphs depict the mean+/−SD of 4 independent experiments. Thrombin induced significant elevations of RhoA and reductions of Rac1 activity as indicated by asterisks (p<0.05 compared to sham). FX06 alone had the reverse effect (asterisks denote p<0.05 compared to sham). In thrombin plus FX06-treated cells, RhoA activation was completely inhibited (asterisks denote p<0.05 compared to cells treated with thrombin alone). The Western blot is an example of a pull down experiment 2 minutes after stimulation.