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Endothelial cells were treated with thrombin, FX06 or thrombin+FX06 for 1 min. Following lysis, proteins were immunoprecipitated (IP) and co-precipitated proteins detected by western blots (WB) as indicated in (A–D). Mean+/−SD of three independent experiments; *p<0.05 compared to medium control, **p<0.05 compared to thrombin; representative Western blots are shown at the right side.
