Figure 4.

Replacement of the γ-globin 3′-untranslated region with its β-globin counterpart in the γ-globin lentiviral vector leads to increased levels of transgene mRNA in peripheral blood reticulocytes. Relative levels of γ-globin mRNA, normalized to bone marrow vector copy, as determined by quantitative reverse transcription–PCR in individual transplanted mice of the indicated groups. Mean ± SEM and the P value are shown. Data were obtained 4–5 months post-transplantation.