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. 2009 Jan 22;329(1):192–201. doi: 10.1124/jpet.108.148916

Fig. 5.

Fig. 5.

Overexpression of murine RORα4 and γ1 dramatically increases endogenous CYP2C8 mRNA in HepG2 cells. Adenoviruses containing FLAG-mRORα4 and the control LacZ (A) or FLAG-mRORγ1 and the control GFP/β-Gal (B) were utilized to infect HepG2 cells in triplicate for 1 to 4 days with a dose of 1000 particles/cell. Cells were harvested and used to isolate total RNA. cDNAs were synthesized with MMLV, and then real-time quantitative PCR analyses were performed to determine the expression of CYP2C8 and TBP. Endogenous CYP2C8 mRNA gradually increased after infection of HepG2 cells with adenoviruses containing ROR over those infected with LacZ significantly (**, p < 0.01; ***, p < 0.001). Data represent means ± S.E. (n = 9). All data are normalized to TBP levels. The same ROR viruses and the GFP control were used to infect HepG2 cells in six-well plate in duplicates. After 2 days, the whole-cell lysates were prepared, and the Western blot was performed to detect the expression of exogenous FLAG-tagged ROR protein (C and D).