Fig. 6.

Overexpression of murine ROR dramatically elevated endogenous CYP2C8 mRNA in human primary hepatocytes. Human primary hepatocytes were infected with adenoviruses for FLAG-mRORα4 (A) and FLAG-mRORγ1 (B) and the control GFP/β-Gal at a dose of 1000 particles/cell. Total RNA was isolated after 60 h, and real-time quantitative PCR analyses were performed to determine the expression of CYP2C8 relative to TBP. Both ROR viruses significantly increased endogenous CYP2C8 mRNA over the GFP adenoviral control (p < 0.001). Data represent means ± S.E. (n = 9). All data are normalized to TBP levels.