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Effects of substrate concentration on the amounts of 5-oxo products formed by microsomal 5-HEDH. Microsomal fractions from PMA-differentiated U937 cells were incubated with different concentrations of 5h-16:2 (▴), Δ6,8,11-5h-20:3 (○), or 5-HETE (•) as described in the legend to Fig. 2, and the amounts of 5-oxo products formed were determined by RP-HPLC (Table 1). The values are from a single experiment and are representative of five independent experiments with similar results.
