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Inhibition of 5-HEDH by 5-hydroxy fatty acids. Microsomal fractions (15 μg/ml) from PMA-differentiated U937 cells were incubated with 5-HETE (1 μM) in the presence of various 5-hydroxy fatty acids (0.3 μM) and NADP+ (100 μM) for 10 min at 37°C. The amounts of 5-oxo-ETE formed were determined by RP-HPLC. The values are means ± S.E. of four independent experiments.
