Fig. 5.
Concentration-response curves for the inhibition by 5-hydroxy fatty acids of 5-oxo-ETE formation by microsomal 5-HEDH. Microsomal fractions (15 μg/ml) from PMA-differentiated U937 cells were preincubated with various concentrations of 5h-18:1 (▴), 5h-20:1 (•), 5h-20:2 (○), 5h-20:0 (▿), and Δ6,8,11-5h-20:3 (5h-20:3, ▾) for 5 min followed by incubation with 5-HETE (1 μM) in the presence of NADP+ (100 μM) for 10 min. The amounts of 5-oxo-ETE formed were determined by RP-HPLC. The values are means ± S.E. (n = 4).