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. 2009 Jan 22;329(1):335–341. doi: 10.1124/jpet.108.143453

Fig. 7.

Fig. 7.

Importance of the α and ω ends of the substrate for metabolism by 5-HEDH. A, time courses for the metabolism of 5-HETE (2 μM) to 5-oxo-ETE (□) and 5-HETE-Me (2 μM) to 5-oxo-ETE-Me (•) by microsomal fractions (30 μg/ml protein) from human neutrophils in the presence of NADP+ (100 μM). B, concentration-response relationships for the effects of 5-HETE-Me (•) and 5,18-diOH-18:2 (▵) on the conversion of 5-HETE (1 μM) to 5-oxo-ETE by neutrophil and U937 cell microsomes, respectively, in the presence of NADP+ (100 μM). Microsomal fractions (30 μg/ml protein) were incubated with 5-hydroxy fatty acids for 15 min. C, time courses for the metabolism of 5-HETE to 5-oxo-ETE (□), 5h-18:2 to 5-oxo-18:2 (○), and 5,18-diOH-18:2 to 5-oxo-18-HODE (•) by microsomal fractions (30 μg/ml protein) from PMA-differentiated U937 cells in the presence of NADP+ (100 μM). All values are means ± S.E. (n = 4).