TABLE 2.
Km and Vmax values for the metabolism of some 5S-hydroxy fatty acids by 5-HEDH-containing U937 cell microsomes Various concentrations of 5-hydroxy fatty acids were incubated with U937 cell microsomes (15 μg/ml protein) for 10 min at 37°C. The amounts of 5-oxo products formed were determined by RP-HPLC as shown in Table 1. The values were determined by nonlinear regression using GraFit software and are means ± S.E. (n = 5).
Substrate | Km | Vmax |
---|---|---|
nM | pmol / min / mg | |
5h-16:2 | 435 ± 114 | 383 ± 42 |
5h-18:2 | 275 ± 99 | 445 ± 59 |
Δ6,8,11-5h-20:3 | 436 ± 158 | 608 ± 66 |
5-HETE | 516 ± 190 | 1062 ± 147 |
5h-20:5 | 679 ± 151 | 872 ± 146 |