Fig. 1.

Effect of l-NAME and NAC on shear-induced HO-1 expression. BAECs were preincubated and sheared (10 dynes/cm², 6 h) in the presence of either l-NAME (1 mM) or NAC (10 mM), and HO-1 protein expression was measured as described under Materials and Methods. Representative immunoblot for HO-1 (32 kDa) and loading control β-actin (42 kDa) and densitometric analysis for levels of HO-1 protein relative to β-actin in static or sheared ECs in presence or absence of l-NAME and NAC are shown. Values are mean ± S.E.M. ^*, significantly different from static control group; P ≤ 0.05; n = 3. †, significantly different from shear group; P ≤ 0.05; n = 3.