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. 2009 Jan 8;329(1):94–101. doi: 10.1124/jpet.108.145557

Fig. 2.

Fig. 2.

Effect of selective antioxidants and ETC inhibitors on shear-induced HO-1 expression. ECs were preincubated and sheared in the presence of ebselen (5 μM), DPI (20 μM), UA (100 μM), myxothiazol (10 μM), rotenone (2 μM), or antimycin A (10 μM), and HO-1 protein expression was measured as before. A, representative immunoblot for HO-1 and β-actin and densitometric analysis for HO-1 protein levels relative to β-actin in static or sheared ECs in the presence or absence of antioxidants ebselen, DPI, and UA (because they had no effect on HO-1 expression in static ECs, only their effects on sheared ECs are shown). B, same as in A but in the presence or absence of ETC inhibitors myxothiazol, rotenone, and antimycin A. Values are mean ± S.E.M. *, significantly different from static control group; P ≤ 0.05; n = 3. †, significantly different from shear group; P ≤ 0.05; n = 3.