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. Author manuscript; available in PMC: 2009 Apr 20.
Published in final edited form as: Mol Cell Proteomics. 2005 Mar 20;4(6):819–826. doi: 10.1074/mcp.T500005-MCP200

Figure 1. Schematics of the Yeast and Bacterial two hybrid systems.

Figure 1

A. In the yeast two-hybrid system shown, a dimeric λ cI-bait hybrid protein interacts with an activation domain (AD)-prey hybrid protein thereby stimulating transcription from an adjacent promoter that directs expression of a quantitative GusA or selectable LYS2 reporter gene. B. In the bacterial two-hybrid system shown, a dimeric λ cI-bait hybrid protein interacts with an E. coli RNA polymerase (RNAP) α-subunit-prey hybrid protein, thereby recruiting RNAP to an adjacent promoter that directs expression of a quantitative lacZ or selectable HIS3 reporter gene. Note that both systems utilize a λ cI-bait hybrid protein from a single plasmid effective in either organism.