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. Author manuscript; available in PMC: 2009 Apr 20.
Published in final edited form as: J Immunol. 2008 Apr 1;180(7):4742–4753. doi: 10.4049/jimmunol.180.7.4742

Figure 7. Naïve CD4+ T cells from CD4Cre/R-DTA mice can be detected shortly after CD4-depletion or when the peripheral T cell pool has been replenished by wild-type T cells.

Figure 7

A) The frequency of CD4+ T cells in the peripheral blood of CD4Cre/R-DTA mice was analyzed in two individual mice at different days after intraperitoneal administration of 200 μg anti-CD4 monoclonal antibody (GK1.5). The experiment was repeated once with similar results. B) Analysis of the frequency of naïve (CD62L+CD44lo) CD4+ T cells at 0, 7, 12 and 18 days after anti-CD4 depletion. C) Percentage of wild-type (Thy1.1+) CD4+ T cells among total donor-derived (Ly5.2+) CD4+ T cells in the peripheral blood at 4, 6 and 9 weeks after bone marrow reconstitution of lethally irradiated Ly5.1+ recipient mice with 106 total bone marrow cells from Thy1.2+Ly5.2+ CD4Cre/R-DTA mice and 105 total bone marrow cells from Thy1.1+Ly5.2+ wild-type mice. D) Left: frequency of total thymocytes derived from wild-type bone marrow (Thy1.1+) or CD4Cre/R-DTA bone marrow (Thy1.1−). Middle: frequency of thymocyte subsets derived from wild-type precursors. Right: frequency of thymocyte subsets derived from CD4Cre/R-DTA precursors. Lower panel: Frequency of naïve (CD44lo) and activated/memory phenotype (CD44hi) CD4+ T cells from wild-type (Thy1.1+) and CD4Cre/R-DTA (Thy1.1−) donors in spleen and lymph node. Data are representative of 4 individual mice. E) Frequency of naïve (CD44lo) CD4+ T cells derived from wild-type bone marrow or CD4Cre/R-DTA bone marrow in mixed bone marrow chimeras (MC) in comparison to the frequency of naïve CD4+ T cells in wild-type and CD4Cre/R-DTA mice.