Fig. 7.
Increased β-cell apoptosis in Tg RIP1-PolγExo(−) mice. Levels of β-cell proliferation and apoptosis were determined by immunostaining for insulin (green) and either 2′-deoxyuridine:5-fluoro-2′-deoxyuridine incorporation (BrdU; red; A) or cleaved caspase-3 (red; C) on sections prepared from wild-type and transgenic mice. B: quantification of BrdU-positive/insulin-positive cells showed no significant difference in β-cell proliferation in the Tg mice compared with WT (n = 3). D: quantification of cleaved caspase-3-positive/insulin-positive cells indicates that there is a 7- and 11-fold increase in the apoptosis of β-cells in 1-wk-old female and male Tg mice, respectively. Less than 0.1% of WT β-cells undergo apoptosis at this age (P < 0.05; n = 4–7).