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. 2009 Feb 3;296(4):E748–E757. doi: 10.1152/ajpendo.90935.2008

Fig. 2.

Fig. 2.

2H NMR spectra of MAG derived from natural abundance glucose (A), medium glucose from myotube culture (B, protocol 3), medium glucose from skeletal muscle tissue incubation (C, protocol 4), and glycogen from skeletal muscle tissue incubation (D, protocol 4). The scale of spectrum (D) is ∼1/8 of the others. Protocol 3 is myotube culture using Dulbecco's PBS (D-PBS) containing 20 mM [U-13C3]lactate, 5% 2H2O, 2% FBS, 5 mM glucose, and 10 mM glutamine. Protocol 4 is muscle tissue incubation using 0.1% BSA, 10 mM glucose, 10 mM glutamine, 20 mM lactate (20% [U-13C3]lactate), and 5% 2H2O.