Table 2.

¹³C NMR analysis of MAG derived from NA glucose, from medium glucose of myotubes, and from medium glucose and skeletal muscle glycogen in tissue incubation

	Glucose (NA)	Myotubes		Minced Skeletal Muscle, Protocol 4
		Protocol 2	Protocol 3	Medium	Glycogen
C2D12	0.0049±0.0003	0.0052±0.0009	0.0053±0.0007	0.0049±0.0005	ND
C2D23	0.0053±0.0005	0.0051±0.0003	0.0053±0.0004	0.0050±0.0005	ND
C2Q	ND	ND	ND	ND (n = 8)	0.4784±0.0788 (1.13)
				0.0009±0.0021 (0.0021)
C5D45	0.0056±0.0004	0.0051±0.0007	0.0054±0.0006	0.0052±0.0005	ND
C5D56	0.0057±0.0003	0.0051±0.0004*	0.0052±0.0004*	0.0051±0.0007	ND
C5Q	ND	0.0057±0.0014 (0.0122)	0.0065±0.0030 (0.0139)	ND (n = 6)	0.6469±0.0441 (1.39)
				0.0014±0.0026 (0.0030)

Values are means ± SE. ¹³C NMR analysis of MAG derived from NA glucose (n = 6), from medium glucose of myotubes (protocols 2–3, n = 6 for each), and from medium glucose (n = 10) and skeletal muscle glycogen (n = 6) in tissue incubation. Each value is the ratio of peak area of each resonance normalized by areas of methyl resonances of MAG on ¹³C NMR spectra (A_{each resonance}/A_methyl). Nos. in parentheses are excess ¹³C enrichment (%). The protocols are described in the text and are summarized in the legend to Table 1. ND, not detectable.

^*Significantly different from NA glucose, P < 0.05.