Fig. 1.

Immunohistochemical analysis of human fatty acid-binding protein 1 (FABP1) transgenic (Tg) mice. Immunohistochemical analysis was performed with the antibody that reacts with mouse and human FABP1. Because of the silencing sequence in the upstream promoter region, mouse FABP1 expression in C57BL/6 wild-type (WT) kidney was absent under normal conditions (A). Renal ischemia-reperfusion (I/R) injury (C) or exposure to cisplatin (CP; E) did not induce FABP1 expression in WT kidney. In human FABP1 Tg mouse kidney, constitutional expression of FABP1 protein was found under normal conditions (B) and FABP1 expression was amplified and expanded in the S3 segment of the cortex 24 h after renal I/R (D). In 20 mg/kg CP-injected Tg kidney (F), FABP1 expression of damaged tubules was diminished or disappeared, whereas viable tubules remained positive 72 h after CP injection. AKI, acute kidney injury.