Fig. 1.

Characterization of skeletal muscle-specific G_sα-knockout (KO) mice (MGsKO). A: immunoblotting analysis of tissue protein extracts from wild-type (E1^+/+), E1^flox/flox:Mck-cre⁻ (Gs^flox/flox), and MGsKO mice using a G_sα-specific antibody. The presence of long and short forms of G_sα produced by alternative splicing of exon 3 results in a doublet. WAT, white adipose tissue. B and C: body weight (B) and body composition (fat and lean mass) (C) of male and female MGsKO (closed bars) and control (open bars) mice (n = 10–16/group). D and E: systolic, diastolic, and mean arterial pressure (MAP) (D) and heart rate (E) in MGsKO mice and controls (bpm, beats/min; n = 5–6/group).