Table 3.

Percentage of NHE3, NaPi2, and associated proteins in the detergent-insoluble fraction

Protein	Window I		Window II		Window III
	Control	High BP	Control	High BP	Control	High BP
NHE3	80±10	90±6	83±3	83±4	76±2§	75±3*†
DPPIV	29±0	38±7	28±4	20±1	24±4	20±3
Myosin VI	100	100	100	100	100	100
Megalin	38±1	51±4	28±1*	31±3*	24±5	23±5*
NaPi2	45±7	59±2	71±3*	68±3	86±4*†	82±1*†
NHERF-1	59±4	76±8	57±6	64±3	58±4	63±3
AP2	78±11	91±7	94±1	93±1	94±1	94±1
Ezrin	52±19	49±14	63±4	60±3	70±14	76±5

Values are means ± SE; n = 5. Fractions after sorbitol density gradient centrifugation from control and acutely hypertensive animals were pooled into 3 windows, adjusted to the same protein concentration and then treated with cold 1% Triton X-100 and centrifuged to separate detergent insoluble pellet from detergent soluble supernatant. The pellet was resuspended in same volume as supernatant. A constant amount of supernatant and resuspended pellet was assayed by immunoblot to determine percentage in detergent insoluble fraction. WI, pooled fractions 4–5; WII, pooled fractions 6–7; WIII, pooled fractions 8–10.

^*P < 0.05 compared with WI;

^†P < 0.05 compared with WII within the same treatment. There was no myosin VI detected in detergent soluble fraction so no error could be calculated.