Fig. 3.
Effects of 5-nitro-2-2(-henylpropylamino) benzoic acid (NPPB) and DIDS on lubiprostone-evoked Isc in guinea pig ileum. NPPB (200 μM), a nonspecific chloride channel blocker, or DIDS (250 μM), a specific inhibitor of cellular anion permeability, was applied into the serosal or mucosal compartment of the Ussing chamber 5 min before adding 300 nM lubiprostone. NPPB abolished lubiprostone-evoked ΔIsc. DIDS did not alter lubiprostone-evoked increases in Isc. DIDS or NPPB was applied 5 min before application of lubiprostone. Values are expressed as means ± SE, n = 6 animals. ***P < 0.001.