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Genotyping of wild-type (SSAT-wt) and SSAT-deficient (SSAT-ko) mice. Genomic DNA was isolated and subjected to PCR amplification by using specific primers to identify the SSAT-wt and SSAT-ko mice. All animals depicted except the heterozygous animal were males. The amplified DNA was size fractionated on a 1% agarose-Tris/acetate/EDTA gel and analyzed for the presence of 408 bp (SSAT-wt) and 680 bp (SSAT-ko) PCR amplification products. Lane marked as Std contains DNA size standard.
