Fig. 2.

Effects of conditioned media from H508 cells (A) and addition of HBEGF to untreated H508 media (B) on proliferation of SNU-C4 human colon cancer cells. A: SNU-C4 cells were incubated with ACh or conditioned media from H508 cells and inhibitors for 5 days at 37°C. B: SNU-C4 cells were incubated with reagents alone in serum-free media or in combination in untreated H508 media. Values are means ± SE of ≥3 separate experiments. *P < 0.05 vs. ACh-stimulated cells. **P < 0.05 vs. cells stimulated by H508 supernatant alone. ***P < 0.05 vs. cells stimulated by HBEGF alone in untreated H508 media.