Fig. 3.
H508 cells express multiple epidermal growth factor (EGF) receptor (EGFR) ligands. A: relative mRNA levels of 3 EGFR ligands were measured by quantitative real-time PCR (Q-PCR). B: ACh-induced H508 cell proliferation is not affected by blocking amphiregulin (AR) with a neutralizing antibody. H508 cells were incubated with ACh alone, recombinant AR (rAR) alone, AR antibody alone, or combined reagents for 5 days at 37°C. Cell proliferation was determined using the CellTiter 96 AQueous One solution assay. TGFα, transforming growth factor-α. Values are means ± SE of 3 separate experiments. *P < 0.05 vs. AR-stimulated cell proliferation.