Fig. 5.

Specific FAK phosphorylation sites selectively control AKT activation by strain. Caco-2 cells were cotransfected with HA-tagged wild-type (wt) or point-mutated FAK constructs and HA-tagged AKT2 and subjected to static or cyclic strain conditions before immunoprecipitation of the HA antigen and immunoblot for phospho-AKT. Cotransfection with FAK Y397F or Y576/577F did not alter strain-induced AKT activation (A, n = 12, *P < 0.03), but FAK Y925F cotransfection blocked strain-induced AKT activation (B, n = 3, *P < 0.05, **P < 0.01). HA, hemagglutinin.