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Effects of upstream kinases on MLC phosphorylation. Caco-2 MLC phosphorylation was not prevented by Src inhibition by PP2 (A, n = 9, *P < 0.002) or Src reduction by siRNA (B, n = 12, *P < 0.05), but reducing FAK (C) or Rac1 (D) by siRNA prevented strain-stimulated MLC phosphorylation (n ≥ 7, *P < 0.01, **P < 0.0001) in Caco-2 cells.
