Fig. 1.

Influence of IL-1β and nitric oxide (NO) on Zip14 expression. A: hepatocytes from inducible NO synthase (iNOS)^−/− and C57BL/6 wild-type (WT) mice were treated with the NO donor IL-1β (100 U/ml) or S-nitroso-N-acetylpenicillamine (SNAP) (100 μM) for 8 h, and Zip14 mRNA was measured by quantitative real-time PCR (qPCR) B: hepatocytes from C57BL/6 mice were exposed to either IL-1β (100 U/ml) or SNAP (100 μM) for up to 16 h. Relative iNOS mRNA abundance was measured. Similar results were achieved with hepatocytes from 129S3/SvImJ mice. C: WT hepatocytes were exposed to IL-1β (100 U/ml) for up to 16 h. The nitrite concentration of the medium in response to IL-1β was measured by using the Griess reaction and normalized to the total cell number per well. D: contribution of metallothionein (MT) to regulation of Zip14 by NO was investigated by incubating MT^−/− and corresponding control strain hepatocytes with or IL-1β (100 U/ml) or SNAP (100 μM) for 8 h. Values are means ± SD of 3 independent experiments. Values with different letters are significantly different (P < 0.001).