Fig. 4.

NO upregulates ZIP14 protein expression in liver parenchymal cells. Hepatocytes from WT (C57BL/6) mice were incubated with SNAP for 8 h. A: total cell lysates were separated by SDS-PAGE, and ZIP14 was detected by Western blot analysis using an affinity-purified ZIP14 antibody. Top: increased ZIP14 expression produced by SNAP. Bottom: tubulin loading control. B: nonpermeabilized primary hepatocytes were stained with 4′,6-diamidino-2-phenylindole (DAPI) for visualization of the nucleus, and an affinity-purified ZIP14 antibody was used for immunolocalization of ZIP14. C: untreated cells were used as a control. Representative images from SNAP-treated and untreated cells using identical gain settings are shown.