Fig. 5.

Fluorescent detection of NO-mediated zinc uptake in hepatocytes from WT and iNOS^−/− mice using FluoZin3-AM. Hepatocytes were treated without (A) or with (B) Il-1β for 8 h, followed by incubation with 40 μM zinc for 5 min, and then immediately visualized by fluorescence microscopy. C: hepatocytes were incubated with IL-1β for 8 h, and then an anti-ZIP14 antibody was added before addition of zinc to the medium. D: hepatocytes were treated as above, but a nonspecific antibody (N/S ab) was used instead of an anti-ZIP14 antibody.