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. 2009 Jan 23;296(4):H1048–H1057. doi: 10.1152/ajpheart.00491.2008

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Fig. 4. — PDBu-elicited increases in force generation, and superoxide are involved in a p47^phox stimulation of Nox2, which is not dependent on extracellular calcium. Effect of calcium on increase in superoxide generation (A) and coronary artery contraction (B) by PDBu is shown. Increase in Nox-derived superoxide generation induced by PDBu is not affected by removing and chelating extracellular calcium with 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-AM (200 μM BAPTA-AM) and ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt (100 μM EGTA). Effect of PDBu on superoxide generation (C and D) and contraction (E) of aorta from gp91^phox and p47^phox knockout mice is demonstrated. Superoxide generation and contraction evoked by PDBu were completely inhibited in endothelium-denuded gp91^phox and p47^phox mouse aorta (n = 6) and by acute treatment with gp91^ds-tat (n = 6). Note that error bars are overlapping with the symbols.