Fig. 7.

Roles for Rho kinase and CaM kinase II pathways in contractions elicited by PDBu. A: inhibition of p38 MAP kinase and ERK1 and 2 by SB202190 (SB; 10 μM) and PD98059 (PD; 10 μM), respectively, did not affect PDBu-induced coronary arterial contraction (n = 15). B: PDBu-induced contraction of coronary arteries was partially inhibited by the Rho kinase inhibitor Y-27632 (10 μM) and the CaM kinase II inhibitor KN-93 (10 μM) and completely by a combination of the two inhibitors (n = 20–25). C and D: hydrogen peroxide scavenging with peg-catalase (150 U/ml) had additive inhibitory effect in combination with Y-27632 (C; n = 8–10) or KN-93 (D; n = 8–10) on contractions evoked by PDBu. E: in mouse aorta, PDBu-induced contraction is completely inhibited by KN-93 (10 μM) but not by Y-27632 (10 μM).