Fig. 5.

Quantitative real-time PCR analysis of mRNA at baseline, after 4 wk of TAC, and 4 and 7 days after TAC reversal. A: expression of genes involved in cardiac hypertrophy [β-myosin heavy chain (MHC), smooth muscle α-actin, and brain natriuretic peptide (BNP)]. B: expression of genes associated with cardiac remodeling [tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, matrix metalloproteinase (MMP)-2, and pro-collagen I]. One-way ANOVA was performed to determine significance, followed by a Holm-Sidak pairwise comparison to significance between groups: §P < 0.05 vs. WT mice at 4 wk of TAC; †P < 0.05 vs. WT and MuRF1^−/− at BL, 4 days of TAC reversal, and 7 days TAC reversal; and *P < 0.001 vs. all other groups. Arrows indicate the reversal of TAC.