Fig. 5.

Representative fluorescent images showing stronger cellular C-H₂DCFDA staining (green fluorescence; an indicator of production of ROS, including H₂O₂) in primary M. musculus fibroblasts (A) than in P. leucopus fibroblasts (C). High glucose treatment elicited substantial increases in DCF fluorescence in M. musculus fibroblasts (B), whereas high glucose-induced increases in mitochondrial ROS generation were significantly lower in P. leucopus fibroblasts (D). Hoechst 33258 (blue fluorescence) was used for nuclear staining. E: bar graphs are summary data of DCF fluorescent intensities in control and high glucose-treated M. musculus and P. leucopus fibroblasts. Data are means ± SE. *P < 0.05. untreated animals; #P < 0.05 vs. M. musculus.