Skip to main content
. 2009 Jan 30;296(4):H946–H956. doi: 10.1152/ajpheart.00693.2008

Fig. 6.

Fig. 6.

A: time course of the build up of MitoSox fluorescence in en face preparations of M. musculus and P. leucopus aortas. Bar graphs are summary data for the slope factor obtained from the time course experiments normalized to tissue mass, representing mitochondrial ROS production in the tissues. Shown are the effects of high glucose treatment (30 mM for 24 h) on mitochondrial ROS production in both species. Data are means ± SE; n = 5 animals/group. *P < 0.05 vs. untreated animals; #P < 0.05 vs. M. musculus. B and C: representative fluorescent images showing stronger perinuclear MitoSox staining (red fluorescence) in high glucose-treated primary M. musculus fibroblasts (B) than in P. leucopus fibroblasts (C). Hoechst 33258 (blue fluorescence) was used for nuclear staining. Scale bars = 50 μm. D: cellular MitoSox fluorescence intensities were assessed using flow cytometry as described in methods. Bar graphs are summary data for mean MitoSox fluorescent intensities. Data are means ± SE. Experiments were performed in quadruplicate with identical results. *P < 0.05 vs. untreated animals; #P < 0.05 vs. M. musculus.