Fig. 7.

High glucose-induced inflammatory gene expression in M. musculus and P. leucopus aortas. A: in cultured aortic segments isolated from M. musculus, high glucose treatment (30 mM for 24 h) elicited significant increases in the mRNA expression of TNF-α, IL-6, ICAM-1, VCAM, and monocyte chemoattractant protein (MCP)-1. B: in contrast, 24 h of high glucose treatment did not result in the significant induction of these inflammatory markers in aortas of P. leucopus. Analysis of mRNA expression was performed by real-time quantitative RT-PCR. β-Actin was used for normalization. Data are means ± SE; n = 5 animals/group. *P < 0.05. vs. untreated animals.