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. 2009 Feb 6;296(4):H1058–H1068. doi: 10.1152/ajpheart.00906.2008

Fig. 1.

Fig. 1.

Microinjection sites in subpostremal nucleus tractus solitarii (NTS) for all experiments. Schematic diagrams show transverse sections of medulla oblongata from a rat brain. AP, area postrema; c, central canal; 10, dorsal motor nucleus of vagus nerve; 12, nucleus of hypoglossal nerve; Ts, tractus solitarius; Gr, gracile nucleus; Cu, cuneate nucleus. Scale is shown at bottom; number on left schematic diagram denotes the rostrocaudal position in millimeters of the section relative to the obex according to the atlas of the rat subpostremal NTS by Barraco et al. (2). Microinjection sites for selective NTS A2a adenosine receptor agonist (CGS-21680; 2.0 and 20 pmol in 50 nl; n = 9 and 8, respectively), selective A2a adenosine receptor antagonist (ZM-241385; 40 pmol in 100 nl; n = 9), nonselective A1 + A2a adenosine receptor antagonist (8-SPT; 1 nmol in 100 nl; n = 9), and respective volume control [artificial cerebrospinal fluid (ACF); 50 and 100 nl; n = 8 and 9, respectively] were marked with fluorescent dye. A: ○, CGS 2.0 pmol; •, CGS 20 pmol; □, ACF 50 nl. B: ▵, ZM; ▴, 8-SPT; ▪, ACF 100 nl.