Table 1.

Summary of Ca²⁺ measurement data

	Control Response, ΔF Ratio	Test Response, ΔF Ratio	Inhibition, %	P Value	n	N
Puromycin
Thapsigargin	0.313±0.061	0.019±0.002	93.9	0*	3	60
Emetine	0.306±0.014	0.119±0.037	61.2	0.0070*	3	32
Anisomycin	0.226±0.012	0.080±0.048	64.6	0.0079*	3	24
Thapsigargin
Emetine	0.341±0.072	0.278±0.058	18.5	0.0012*	5	36
Puromycin	0.274±0.062	0.294±0.068	−7.3	0.5829	5	28
Anisomycin (200 μM)	0.262±0.048	0.273±0.032	−4.2	0.4307	3	24
Anisomycin	0.211±0.086	0.197±0.079	6.5	0.1416	3	36
Anisomycin (37°C)	0.244±0.017	0.249±0.030	−2.0	0.8963	5	76
Anisomycin (1.5 mM Ca2+, 37°C)	0.183±0.038	0.155±0.054	15.5	0.6823	4	48
Anisomycin (10 mM Ca2+, 37°C)	0.166±0.040	0.150±0.031	9.8	0.7599	4	48
Ionomycin
Emetine	0.669±0.065	0.608±0.065	9.2	0.0967	3	18
Puromycin	0.654±0.046	0.681±0.046	−4.1	0.7671	3	18
Anisomycin	1.264±0.289	1.148±0.233	9.2	0.0732	3	40
Anisomycin (37°C)	0.502±0.087	0.383±0.077	23.7	0.0725	4	60
ATP
Emetine	0.214±0.081	0.207±0.060	3.3	0.7546	4	64
Anisomycin	0.226±0.077	0.235±0.095	−3.2	0.8792	3	24
Puromycin	0.323±0.028	0.253±0.087	21.8	0.6235	3	24
Endothelin-1
Emetine	0.504±0.186	0.466±0.058	7.5	0.6530	3	44

Values are means ± SE; n, number of independent experiments; N, number of wells of the 96-well plate. Puromycin, thapsigargin, emetine, ionomycin, ATP, and endothelin-1 were used at 200, 3, 40, 5, 100 and 0.1 μM, respectively. Anisomycin was used at 400 μM unless indicated. Experiments were performed in the absence of extracellular Ca²⁺ and at room temperature, unless at 37°C as indicated. Preincubation with inhibitors was in the absence of Ca²⁺ for 1 h unless the preincubation included 1.5 or 10 mM Ca²⁺ where indicated. ΔF, change in fluorescence.

^*P < 0.05.