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(A) MALDI mass spectrum of 1 pmol of FGF-2. The theoretical m/z value of [M+H]+ for FGF-2 is 17,102. Present in the spectrum are dimers, trimers, tetramers, and pentamers. Also present is a peak at m/z 25,640 corresponding to a doubly charged species of the trimer. (B) MALDI mass spectrum of FGF-1. The theoretical m/z value of [M+H]+ for FGF-1 is 15,899. Present in the spectrum is only a weak dimer peak. The instrument was calibrated externally with myoglobin and BSA.
