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. 1999 Mar 2;96(5):1892–1897. doi: 10.1073/pnas.96.5.1892

Figure 4.

Figure 4

Representative blots of (a) FGF-1 or (c) FGF-2 crosslinking reactions. (b) Gel showing the purity of the uncrosslinked monomer of FGF-1 and FGF-2 (left and right, respectively). (a and c) The protein/heparin ratio was held constant at 10:1. Concentrations of FGF-1 in a are 240, 180, 120, 60, and 30 μM, left to right. For FGF-2 in c, the concentrations crosslinked are 30, 60, 120, and 180 μM, left to right. The blot of FGF-1 is overstained to demonstrate the clear lack of higher-order oligomers in the crosslinked products.