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. 2009 Jan 30;296(4):H976–H986. doi: 10.1152/ajpheart.01134.2008

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Fig. 7. — Fibrosis and TUNEL analysis in transplanted and recipient hearts. A–D: collagen deposits (dark red brick color) indicated by picrosirius red staining under polarized light microscopy at 3 wk in transplanted (A and B) and recipient (C and D) hearts. Yellow, viable myocardium. Magnification: ×200. E–H: TUNEL-positive nuclei in transplanted (E and F) and recipient (G and H) hearts. Large numbers of TUNEL-positive nuclei (green; white arrows) in transplanted (E) and recipient (G) hearts of the null control group were observed compared with SDF-1α-treated transplanted (F) and recipient (H) hearts. Red, α-sarcomeric actin (for cardiomyocytes); blue, DAPI (for all nuclei). Scale bar = 100 μm. Magnification: ×200. I and J: quantitative estimate of TUNEL-positive nuclei in transplanted (I) and recipient (J) hearts. Data are means ± SE; n = 6 hearts/group. *P < 0.05 vs. the null group.