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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2009 Jan 3;123(2):342–351. doi: 10.1016/j.jaci.2008.11.004

Figure 4. PE-induced shock depends more on macrophages and basophils than on mast cells but is accompanied by mast cell activation.

Figure 4

A. BALB/c mice were treated with saline (no macrophage depletion), or with gadolinium, or clodronate-containing liposomes to deplete macrophages; then pre-treated with IL-4C and propranolol, injected i.v. with 250 μg of PE and followed by rectal thermometry. Serum from mice injected with saline or PE was analyzed for MMCP1 content by ELISA. B. BALB/c mice were treated with gadolinium to deplete macrophages, 35 μg of Ba103 anti-basophil mAb to deplete basophils, both gadolinium and Ba103, or a control mAb isotype-matched to Ba103. Mice were then pre-treated with IL-4C and propranolol, injected i.v. with 250 μg of PE and followed by rectal thermometry. C. BALB/c mice were treated for 15 days with 0.5mg/mouse of anti-c-kit mAb by intraperitoneal injection every other day to deplete mast cells or an isotype-matched control mAb, then pre-treated with IL-4C and propranolol and challenged i.v. with 250 μg of PE. Sera obtained from mice after injection of an activating anti-IgE mAb were assayed for MMCP1 content or histamine content by ELISA. D. BALB/c mice, treated with saline or anti-c-kit mAb as in “B,” were challenged with saline or PE and bled 5 min later. Sera were assayed for histamine content by ELISA. * signifies p <0.05 as compared to saline in “A” and “C” and control mAb in “B.” † signifies p <0.05 for anti-c-kit mAb as compared to saline in “C.”