Fig. 2.

Increased nitrotyrosine staining in lung of newborn Nrf2^−/− mice exposed to hyperoxia. Top: representative example of nitrotyrosine staining from lung of 4-day-old Nrf2^+/+ and 4-day-old Nrf2^−/− mice exposed to 72 h of hyperoxia. Arrows point to nitrotyrosine staining. Bottom: significant increase in nitrotyrosine staining in lung of Nrf2^−/− oxygen-exposed mice compared with Nrf2^+/+ oxygen-exposed mice (P < 0.002) and Nrf2^−/− room air mice (P < 0.004), n = 3 for each group. *Significant difference by P value using Student's t-test. RA, room air.