In vivo knockdown of HIMF reduces hypoxia-induced changes in hemodynamics. Vertical bars represent means ± SE of mean pulmonary artery pressure (mPAP; A), pulmonary vascular resistance (PVR; B), right ventricular end systolic pressure (RVESP; C), right ventricular end diastolic pressure (RVEDP; D), cardiac output (CO; E), and left ventricular end diastolic pressure (LVEDP; F) in rats pretreated with Ad-Neg-shRNA or Ad-HIMF-shRNA and exposed to either normoxia or hypoxia for 14 days. The number of rats studied is indicated within each bar. **P < 0.001 compared with normoxia+Ad-miNeg-shRNA control; ‡P < 0.01 compared with hypoxia+Ad-Neg-shRNA control. ‡‡P < 0.001 compared with hypoxia+Ad-Neg-shRNA control.