Fig. 5.

Effectiveness of pulmonary HIMF gene transfer. A: 2 wk after rats were intratracheally instilled with AAV-HIMF, the lungs were homogenized, resolved by 4–20% SDS-PAGE, and transferred to nitrocellulose. The blots were probed with rabbit anti-HIMF antibodies and developed by ECL. To confirm equal loading and transfer, the blot was stripped and reprobed with anti-β-actin monoclonal antibodies. B: laser densitometry was used to quantify HIMF levels in the lung samples. Data were normalized to β-actin expression and expressed as relative intensity (means ± SE). The number of lungs studied is indicated within each bar. *P < 0.05 compared with the control lung. C–E: paraffin-embedded lung sections from AAV-null-treated (C) and AAV-HIMF-treated (D and E) mice were rehydrated and stained with goat anti-mouse HIMF polyclonal antibodies. Scale bar = 50 μm.