Table 4.
Previously published knockout alleles in the chromosome 4 (117–281) balancer
| Gene | Published phenotype |
| Pre-implantation | |
|
Cdca8 Cdc20 Faf1 |
embryos present at E3.5 but fail to form fully expanded blastocyst [8] embryos arrested at the two cell stage [39] embryos arrested at the two cell stage [12] |
| Nasp | at E3.5, blastocyts are smaller with fewer cells [13] |
| E5.5–E8.5 | |
| Foxd3 | failure to gastrulate, finger-like extensions seen at distal tip [14] |
| Gjb3 | lethality before somite formation [15,16] |
| Hdac1 | embryonic lethality before turning of embryo, growth retarded after E7.5 [17,18] |
| Macf1 | lethality before somite formation [19] |
| Ncdn | resorption was evident prior to E6.5 [20] |
| Urod | lethality before somite formation, no fetuses at E7.5–E9.5 [21] |
| E9.5–E12.5 | |
| Bmp8b | smaller than expected, delayed, die by E9.25 [22] |
| Ppap2b | abnormal gastrulation, dead by E10.5 [23,24] |
| Slc2a1 | prenatal lethality E10–E14, reduced embryo size, overall developmental delay [25] |
| Stil | lethality around E10.5, abnormal left-right axis patterning, growth retardation abnormal neural tube morphology and development [26] |
| Tal1 | lethality at E8.5–E10.5, arrested development, cardiovascular defects, defects in |
| hematopoiesis, distended pericardial sacs, pale and necrotic [9] | |
| E13.5–E18.5 | |
| Dhcr24 | some die before birth, others smaller with less adipose tissue, decreased cholesterol levels, male and female infertility [27] |
| Gjb5 | death at E11.5–E14.5, smaller with placental defects [28] |
| Marcksl1 | neural tube [29] |
| Mtf1 | embryos die around E14 due to liver degeneration [30] |
| Ssbp3 | prenatal lethality, abnormal head development [31] |
| Tie1 | death before E13.5, respiratory, cardiovascular, skin defects [32] |
| Ybx1 | lethality E13.5-just after birth, reduced embryo size, craniofacial defects, abnormal neural tube closure [33] |
| Peri-natal | |
| Jak1 | neonates smaller with abnormal B and T cell development [34] |
| Khdrbs1 | 2/3 die at birth, and the survivors live well into adulthood [35] |
| Nfia | lethality at P0, survivors have severe brain abnormalities [36] |
| Pou3f1 | die within a few hours of birth due to respiratory defects [37] |
| Ror1 | die just after birth with abnormal blood chemistry and respiratory system defects [38] |
| Slc6a9 | die just after birth with severe motor and respiratory defects[40] |
| Post-natal | |
| Dab1 | lethality at 20–30 days, decreased leptin levels, nervous system defects including ataxia, abnormal brain morphology [10,11] |
| Dmbx1 | lethality before P9, smaller, little or no milk in stomachs [7,41,42] |